Application of PRRS Vaccine
Related Product: Xinlanjing -Porcine Reproductive and Respiratory Syndrome Inactivated Vaccine (Strain CH-1a)
1. FABE of Xinlanjing products
(1) 4 major product features
1. Cell suspension culture process
2. Antigen concentration and purification process
3. Serum-free high-titer culture
4. Contains immune booster ingredients
(2) 4 major product advantages
1. The antigen is highly pure, and the immune side effects are slight, which improves the safety of emergency immunization
2. High safety, fast immune response, establish dual protection of cells and body fluids
3. Wide protection spectrum, protected against classic, HP PRRSV and NADC30-like strains
4. Compound TCL (effector T cell) protein epitopes to activate cellular immunity
(3) Three major customer values
(1) Regular vaccination, it is safe, and does not affect the normal production of pigs;
(2) After vaccination in PRRSV positive farms, the clinical symptoms of PRRS can be alleviated;
①Reduce lung damage caused by PRRS;
②Shorten the duration of respiratory diseases;
③Reduce the abortion rate of sows due to PRRS;
④Shorten the duration of fever in piglets;
⑤Reduce the death rate of piglets caused by blue ear
(3) Reduce the activity of blue ear and keep blue ear stable for a long time;
(4) Suggested immunization procedures for different pig farms
Breed of pigs | Positive Stable farm | Positive unstable/onset farm | negative farm |
boar | For the first time, Xinlanjing 2ml/pig will be exempted, and boost once 2 weeks later, and then it will be immunized every 3 months, 2ml/pig | ||
gilt | ① Before mating, immunize with 1 dose of Xinlanjing/pig, boost immunization with live vaccine 1 dose/pig at an interval of 3 weeks, and immunize with 2ml/pig of Xinlanjing after an interval of 2 weeks; ②For farms that live PRRSV vaccines had never been used, use Xinlanning live vaccine 2ml/pig before breeding , and strengthen once 2 weeks later. | It is not recommended to only vaccinate inactivated blue ear vaccine; if it is necessary to vaccinate inactivated blue ear vaccine, it is recommended that the pigs be domesticated with live virus or live vaccine before being immunized with inactivated vaccine; | |
Multiparous sow | Follow-up fetus vaccination: Immunize 1 dose/pig of Xinlanning 2 weeks after delivery, and immunize 2ml/pig of Xinlanjing 8 weeks before delivery; General immunization: Xinlanjing 2ml/pig for the first time, boost once 2 weeks later, and then immunized every 3 months with 2ml/pig | General vaccination : Xinlanjing 1 dose, boost once 3 weeks later; Adopt a stable farm prevention and control plan after stabilization | |
piglet | 14 days old immunize 1 dose of Xinlanning , 28 days old 1ml Xinlanjing | 10-14 days old, 1 dose of Xinlanning ; 28 days old , immunize Xinlanjing 1ml/pig. | Inactivated vaccines are not recommended for immunization |
Drug Care Program: 20% tilmicosin 2Kg + lentinan 500g/t mixed with feed. Positive stable farm: use for 15 consecutive days, once every 2 months . Positive instability/onset farm: once a month for 3 consecutive months, once every 2 months after stabilization, 15 days each time; Before vaccination, use this program for health care of herds and then immunization. | |||
2. The comparative advantages of Xinlanjing
Representative manufacturer | virus strain | Product name | usage plan | Propaganda Features | Xinlanjing 's comparative advantage |
Mebolo | CH-1a strain | Lan Liding | Piglets at the age of 2 weeks are immunized with 1ml for the first time, and boost once 2 weeks later, 1ml/time; male and female pigs are immunized once 2 weeks after the first immunization , and once every 3 months thereafter, 2ml/time; the backup is immunized twice before breeding, 2 weeks apart, 2ml/time | Autophagosomes enhance the cellular immunity of inactivated vaccines | 1. Live plus killed plan; 2. Stimulate cellular immunity through TCL cell (effector T cell) protein epitopes; 3. High cost performance |
Uben | CH-1a strain | You Sapphire | 1ml for piglets between 21day-age and weaning; vaccinate 4 times/year for boars; for outsourced gilts, vaccinate on the day of introduction and boost 2ml/pig 3 weeks later; self-retained gilts, vaccinate at 10-14 day-age and the weaning day for 1ml/pig each, 140-160 day-age+180-200 day-age 2ml/pig | Uplus complex adjuvant; high antigen concentration and purity; | 1. Live plus killed plan; 2. It has the function of activating cellular immunity; 3. High cost performance |
Note: Other CH-1a strain inactivated vaccine manufacturers: Sichuan Hailinger, Harbin Veken Biotech, and Yangzhou Youbang
FAQ for the XINLANNING
1. What are the similarities and differences between Xinlanjing and Mebolo's inactivated blue ear vaccine in terms of product and marketing?
(1) The vaccine approval numbers of the two strains are both CH-1a strains;
(2) Both can activate cellular immunity. Mebolo uses autophagosomes to activate cellular immunity, and Xinlanjing uses TCL cell (effector T cell) protein epitopes to stimulate cellular immunity;
Note: The killing of virus-susceptible cells by effector T cells is the main mechanism of the cellular immune response
(3) Xinlanning and Xinlanjing's "live plus killed" scheme can adapt to more application scenarios.
(4) Sinder obtained the NADC30 and NADC34 strains from the PRRS research group of Harbin Veterinary Research Institute, which are more compatible with the popular strains.
(5) Since the cost of educating customers is low, Sinder's Xinlanning is more cost-effective
(6) Sinder will build PRRS vaccine as a large single product, and will launch NADC30 live vaccine and matching antibody differential diagnosis kit in the future, which will have continuity in product iteration.
2. What are the similarities and differences between Xinlanjing and Uben's PRRS inactivated vaccines in terms of product and marketing?
(1) Both have the "live + killed" immunity method;
(2) The strains with the approval numbers of both vaccines are both CH-1a strains
(3) Youben vaccine uses Uplus adjuvant to enhance humoral immunity. In addition to improving humoral immunity, Xinlanning’s adjuvant also uses TCL cell (effector T cell) protein epitopes to stimulate cellular immunity ;
(4) Sinder obtained the NADC30 and NADC34 strains from the PRRS research group of Harbin Veterinary Research Institute, which are more compatible with the popular strains.
(5) Due to the low cost of educating customers, Sinder XinLanning has a higher cost performance
(6) Sinder will build PRRS vaccine as a large single product, and will launch NADC30 live vaccine and matching antibody differential diagnosis kit in the future, which will have continuity in product iteration.
3. When the high fever occurred in 2008, the pig farm also used inactivated vaccines. But the more immunized, the more the death. Are the current inactivated vaccines effective?
(1) The inactivated PRRS vaccine cannot be used to immunize pigs that have not been infected with live virus. Only the pigs infected with live virus or vaccinated with live vaccine can have the immune memory cells of cellular immunity and humoral immunity related to neutralizing antibodies in pigs. Then immunization with inactivated vaccines can achieve the effect of strengthening immunity and increasing neutralizing antibodies.
(2) If negative pigs are immunized with PRRS inactivated vaccine (especially pigs that have not been domesticated to PRRS), since there is no live virus to initiate the production of neutralizing antibodies, the inactivated vaccine can only produce non-neutralizing hybrid antibodies.
(3) In the immune mechanism of PRRS, there is an ADE effect, and the non-neutralizing hybrid antibody by the inactivated vaccine alone can enhance the infection efficiency of wild virus infection.
(Note: ADE effect: Virus infection starts from adhesion to the cell surface, and adhesion is accomplished through the interaction of virus surface proteins with specific receptors and ligand molecules on target cells. Specificity for virus surface proteins Antibodies can often block this step, "neutralizing" the virus, making it incapable of infecting cells. In some cases, however, non-neutralizing antibodies have the opposite effect during viral infection: they assist The virus enters the target cell and increases the infection rate, this phenomenon is antibody-dependent enhancement)
(4) The high fever that occurred between 2006 and 2010 was caused by the highly pathogenic PRRS virus, which has a very high lethality rate for sows and commercial pigs. And before 2006, the proportion of pigs infected with PRRS in China was relatively low. Most pig farms did not immunize with live vaccines. Prevention and control of PRRS using inactivated vaccine didn’t work. On the contrary, ADE is more easily to occur when infected with wild virus.
(5) Another situation is to immunize inactivated vaccines at the time of onset. Due to the rapid onset and high fatality rate of highly pathogenic PRRS virus, it is too late for the vaccine to work, and large-scale deaths of pigs will occur.
(6) At present, the virulence of PRRS virus in my country is gradually weakening, and it is difficult to see death or severe clinical symptoms in sows infected with PRRS. Moreover, the infection rate of the wild virus in the population is relatively high, so that the reimmunization of such pigs with inactivated vaccines can play the role of inactivated vaccines on the basis of live virus infection.
(7) The effect of inactivated vaccines is positive, and there are many commercial products abroad. Such as the P120 strain of Boehringer in Germany, the European strain approved for use in the United Kingdom and the Czech Republic, and the American inactivated vaccine used in South Korea. Among them, South Korea, the United Kingdom and the Czech Republic have only approved the use of PRRS inactivated vaccines, and PRRS in these countries are relatively less compared to other countries.
4. Isn’t the inactivated vaccine canceled after a short period of approval and application in my country through the green channel? The live vaccine that was launched later, does it mean that the inactivated vaccine is ineffective?
(1) Please refer to item 4/5/6/7/8 of the previous question
The general idea of the answer is: the virulence of the HP PRRSV strain is too high, and the lethality rate is too high, so the inactivated vaccine does not work during the outbreak of high fever; because the foreign strains are relatively mild, clinically, the inactivated vaccine can only produce protective effect timely and conditionally. At present, the overall strains in our country tend to be at a stage where the virulence of the strains is constantly weakening.
(2)PRRS inactivated strain CH-1a has passed the approval of the State Food and Drug Administration in accordance with the standards of new veterinary drugs, and it has been strictly reviewed, not a green channel. The NVDC-JXA1 strain was approved through the green channel.
5. What is your technical plan for PRRS prevention and promotion strategy?
(1) For the onset farm: we recommend emergency immunization with inactivated vaccines, and at the same time use macrolides to control the activity of PRRS, and strengthen the drug health care of piglets
(2) For PRRS positive sites: We give priority to immunization of PRRS inactivated vaccines to reduce the activity of PRRS virus.
(3) For pig farms with stable positive and with need to maintain stable positive for a long time: we use the live PRRS vaccine to reduce the dose and reduce the frequency of immunization in a timely manner , so that the pigs can maintain the immune memory of the PRRS virus, and then use the inactivated vaccine to increase the neutralizing antibody, and reduce the activity of blue ear virus;
(4) For pig farms with stable positivity and need to do closed purification of PRRS: We use live vaccines to immunize the whole herd, so that the pigs can be infected and shed virus at the same time, and then strengthen the inactivated vaccine one month later to reduce the activity of PRRS. Finally, gradually stop using any PRRS live vaccines to achieve group closure and purification .
(5) Negative farms with good biosafety conditions (and can guarantee PRRS negative in introduced breeding pigs): it is not recommended to vaccinate any PRRS live vaccine or inactivated vaccine.
6. Will the immunization of Xinlanjing cause stress in the pig herd? What is the cause of the stress?
The stress after immunization of pigs is mainly caused by two reasons, one is the production process of the vaccine, and the other is the personnel operation specification during immunization.
(1) The cell suspension cultured production process, and the purification process optimized the purity, minimized tress to pigs.
(2) During immunization, do a good job of fully warming up the vaccine before use according to the product requirements; during the immunization process, personnel should operate gently, especially to avoid unexpected actions, and at the same time choose needles with appropriate specifications and quality to prevent accidents such as leakage and multiple supplements during immunization which will increase the frequency of stress ;
(3) After the immunization, the feeding amount can be appropriately reduced to reduce the burden on the body of the gastrointestinal tract; multivitamins can be added to drinking water to reduce the stress response.